Hotspot

trnN-GUU-rpl32

SSC · 110569-114754 · 4186 bp

Back to Diplolaena

Pi 0.00645
MarkerSeek score 54.30
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
4186
variable_sites
27
pi
0.00645
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 CACCGGCTCTTAGCTCTTTT AGTGTAAAGTGTAATTAGGGAA 50.0 31.8 57.9 52.4 233 bp 1.0 41.7
trnN-GUU-rpl32_p2 AATTCACCGGCTCTTAGCTC AGTGTAAAGTGTAATTAGGGAA 50.0 31.8 57.7 52.4 237 bp 1.0 41.7
trnN-GUU-rpl32_p3 CACCGGCTCTTAGCTCTTTT AGTGTAAAGTGTAATTAGGGAAT 50.0 30.4 57.9 53.1 233 bp 1.0 41.7
trnN-GUU-rpl32_p4 ATTCACCGGCTCTTAGCTCT AGTGTAAAGTGTAATTAGGGAA 50.0 31.8 58.5 52.4 236 bp 1.0 41.7
trnN-GUU-rpl32_p5 AATTCACCGGCTCTTAGCTC AGTGTAAAGTGTAATTAGGGAAT 50.0 30.4 57.7 53.1 237 bp 1.0 41.7