Hotspot

rps3-rpl22

LSC · 84791-85133 · 343 bp

Back to Diplolaena

Pi 0.255
MarkerSeek score 89.60
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
343
variable_sites
84
pi
0.255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 TGTTTTGCAAACCAAAGCGA GTTCTTACGAGGAAGCCCTT 40.0 50.0 57.6 57.2 449-599 bp 1.0 81.1
rps3-rpl22_p2 CCAAGTCGAAAACCAAGTGG GTTCTTACGAGGAAGCCCTT 50.0 50.0 57.2 57.2 410-560 bp 1.0 78.0
rps3-rpl22_p3 TTGCAAACCAAAGCGAATGA GTTCTTACGAGGAAGCCCTT 40.0 50.0 57.1 57.2 445-595 bp 1.0 77.0
rps3-rpl22_p4 GCAAACCAAAGCGAATGATGA GTTCTTACGAGGAAGCCCTT 42.9 50.0 58.1 57.2 443-593 bp 1.0 75.6
rps3-rpl22_p5 TGCAAACCAAAGCGAATGAT GTTCTTACGAGGAAGCCCTT 40.0 50.0 56.9 57.2 444-594 bp 1.0 75.2