Hotspot

psbE-petL

LSC · 65960-67342 · 1383 bp

Back to Diplolaena

Pi 0.0116

MarkerSeek score 64.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 16
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.9	56.7	1530-1531 bp	1.0	70.5
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.9	56.7	1529-1530 bp	1.0	70.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGGAGAAAACCA	50.0	45.0	57.8	56.7	1453-1454 bp	1.0	69.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGGAGAAAACCAA	50.0	45.0	57.8	56.7	1452-1453 bp	1.0	69.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGCTAGGAGAAAACCAA	45.0	45.0	58.2	56.7	1538-1539 bp	1.0	69.5