Hotspot

trnM-CAU-atpE

LSC · 53475-53708 · 234 bp

Back to Diplolaena

Pi 0.0214

MarkerSeek score 64.80

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 5
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	330 bp	1.0	77.7
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	AGGACACGAGTCGAGGTTAT	50.0	50.0	58.4	57.9	316 bp	1.0	77.0
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	329 bp	1.0	75.8
trnM-CAU-atpE_p4	GTATTGCTTTCATACGGCGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.3	58.0	331 bp	1.0	75.7
trnM-CAU-atpE_p5	GCGGGAGTCATTGGTTCAAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.8	58.0	315 bp	1.0	75.0