Hotspot

trnF-GAA-ndhJ

LSC · 49936-50400 · 465 bp

Back to Diplolaena

Pi 0.0134

MarkerSeek score 64.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 465
variable_sites: 6
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.0	58.0	602-617 bp	1.0	82.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	566-581 bp	1.0	82.6
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AATGCCGGAAAGCTGGATAG	55.0	50.0	57.9	58.0	590-605 bp	1.0	81.8
trnF-GAA-ndhJ_p4	GTCGGAAATGGTCGGGATAG	AATGCCGGAAAGCTGGATAG	55.0	50.0	57.8	58.0	615-630 bp	1.0	81.5
trnF-GAA-ndhJ_p5	GGAAATGGTCGGGATAGCTC	AATGCCGGAAAGCTGGATAG	55.0	50.0	57.8	58.0	612-627 bp	1.0	81.4