Hotspot

rps2-rpoC2

LSC · 16714-16937 · 224 bp

Back to Diplolaena

Pi 0.0179

MarkerSeek score 62.80

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 4
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCTTCCATCATCTCTTCC	ACAGGGAATTTATTCGGGGG	52.4	50.0	58.2	57.3	376-379 bp	1.0	65.7
rps2-rpoC2_p2	CATTTCCGAGTACCATGACCA	ACAGGGAATTTATTCGGGGG	47.6	50.0	57.7	57.3	406-409 bp	1.0	64.7
rps2-rpoC2_p3	CCTGCTTCCATCATCTCTTCC	AACAGGGAATTTATTCGGGGG	52.4	47.6	58.2	58.0	377-380 bp	1.0	63.4
rps2-rpoC2_p4	CCATTTCCGAGTACCATGACC	ACAGGGAATTTATTCGGGGG	52.4	50.0	58.4	57.3	407-410 bp	1.0	63.3
rps2-rpoC2_p5	TCCGAGTACCATGACCAAAA	ACAGGGAATTTATTCGGGGG	45.0	50.0	56.5	57.3	402-405 bp	1.0	62.6