Hotspot

trnK-UUU-rps16

LSC · 4434-4988 · 555 bp

Back to Diplolaena

Pi 0.033

MarkerSeek score 63.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 18
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	660-675 bp	1.0	83.1
trnK-UUU-rps16_p2	AATTGGAACCCTAGGGCTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	908-923 bp	1.0	82.3
trnK-UUU-rps16_p3	AGCCGTACTTAAAAGCCGAG	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	833-848 bp	1.0	82.1
trnK-UUU-rps16_p4	AGCCGTACTTAAAAGCCGAG	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	834-849 bp	1.0	82.1
trnK-UUU-rps16_p5	GGTAAGGAAGTTAGTGGGCG	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	58.0	58.0	1348-1364 bp	1.0	81.7