Hotspot

trnR-ACG-trnT-UGU

Genome · 148870-149643 · 774 bp

Back to Diplazium

Pi 0.048

MarkerSeek score 83.00

Primer available yes

Label trnR-ACG-trnT-UGU

Feature Metadata

label_name: trnR-ACG-trnT-UGU
feature_type: igs
region: Genome
length_bp: 774
variable_sites: 64
pi: 0.048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnT-UGU_p1	CTCAGGGGATTAGAGCACGT	AGTACGGAGGTTCGAATCCT	55.0	50.0	58.9	57.9	826-869 bp	1.0	81.8
trnR-ACG-trnT-UGU_p2	TCAGGGGATTAGAGCACGTG	AGTACGGAGGTTCGAATCCT	55.0	50.0	59.2	57.9	825-868 bp	1.0	79.5
trnR-ACG-trnT-UGU_p3	TCAGGGGATTAGAGCACGT	AGTACGGAGGTTCGAATCCT	52.6	50.0	57.7	57.9	825-868 bp	1.0	78.5
trnR-ACG-trnT-UGU_p4	CAGGGGATTAGAGCACGTG	AGTACGGAGGTTCGAATCCT	57.9	50.0	57.6	57.9	824-867 bp	1.0	78.0
trnR-ACG-trnT-UGU_p5	CTCAGGGGATTAGAGCACGT	CGTAGTACGGAGGTTCGAATC	55.0	52.4	58.9	58.0	829-872 bp	1.0	74.8