Hotspot

rpl32-trnP-GGG

Genome · 109393-110102 · 710 bp

Back to Diplazium

Pi 0.151

MarkerSeek score 83.40

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 710
variable_sites: 180
pi: 0.151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	TCCCTGCTTGTGCTGATTTT	TATGTAGCGGAATATGGCGC	45.0	50.0	58.0	58.0	1081-1134 bp	0.75	80.4
rpl32-trnP-GGG_p2	TCCCTGCTTGTGCTGATTTT	GAGTACCAAAGTGGCGAGAG	45.0	55.0	58.0	58.0	1233 bp	0.25	50.0
rpl32-trnP-GGG_p3	TCCCTGCTTGTGCTGATTTT	GTACCAAAGTGGCGAGAGAG	45.0	55.0	58.0	58.0	1231 bp	0.25	50.0
rpl32-trnP-GGG_p4	TTGCATTTCTCCCTGCTTGT	GAGTACCAAAGTGGCGAGAG	45.0	55.0	58.0	58.0	1242 bp	0.25	50.0
rpl32-trnP-GGG_p5	TTGCATTTCTCCCTGCTTGT	GTACCAAAGTGGCGAGAGAG	45.0	55.0	58.0	58.0	1240 bp	0.25	50.0