Hotspot

rpl23-trnI-CAU

Genome · 82176-82353 · 178 bp

Back to Diplazium

Pi 0.189

MarkerSeek score 76.70

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: Genome
length_bp: 178
variable_sites: 59
pi: 0.189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	AATGAAAGCGTTGCGATTGG	TGGCGAATTCACAGGTTCAA	45.0	45.0	58.0	58.0	820-827 bp	0.5	66.9
rpl23-trnI-CAU_p2	AATGAAAGCGTTGCGATTGG	TTCCTAGCGGATTTGACGTG	45.0	50.0	58.0	58.0	941 bp	0.25	49.8
rpl23-trnI-CAU_p3	AATGAAAGCGTTGCGATTGG	GTTCCTAGCGGATTTGACGT	45.0	50.0	58.0	58.0	942 bp	0.25	49.8
rpl23-trnI-CAU_p4	TCAGAGGTAGGGCATTTCCA	TTCCTAGCGGATTTGACGTG	50.0	50.0	58.0	58.0	1065 bp	0.25	49.7
rpl23-trnI-CAU_p5	TCAGAGGTAGGGCATTTCCA	GTTCCTAGCGGATTTGACGT	50.0	50.0	58.0	58.0	1066 bp	0.25	49.7