Hotspot

psbE-petL

Genome · 61273-62030 · 758 bp

Back to Diplazium

Pi 0.0727

MarkerSeek score 81.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 758
variable_sites: 97
pi: 0.0727
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	CGAAAATGAAAAGGGGACGC	55.0	50.0	58.1	58.0	1490-1501 bp	0.5	66.4
psbE-petL_p2	ACCTATCAACCACGAGGGAA	TGTTTGAGCTGTGACAACGA	50.0	45.0	58.1	58.0	1839 bp	0.25	49.4
psbE-petL_p3	GCGTTCTCCTGTATTCCCAG	TGTTTGAGCTGTGACAACGA	55.0	45.0	58.1	58.0	1186 bp	0.25	49.4
psbE-petL_p4	ACCTATCAACCACGAGGGAA	CGAAAATGAAAAGGGGACGC	50.0	50.0	58.1	58.0	2154 bp	0.25	49.4
psbE-petL_p5	ACCTATCAACCACGAGGGAA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	2103 bp	0.25	48.9