Hotspot

rps4-trnL-UAA

Genome · 44790-45434 · 645 bp

Back to Diplazium

Pi 0.0987

MarkerSeek score 81.70

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 645
variable_sites: 116
pi: 0.0987
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	CTCAGCGCATTACAGGAAGT	ACGTCTCCATACCTAGGCTC	50.0	55.0	58.0	58.0	1166-1336 bp	1.0	88.8
rps4-trnL-UAA_p2	CTCAGCGCATTACAGGAAGT	CGTCTCCATACCTAGGCTCA	50.0	55.0	58.0	58.0	1165-1335 bp	1.0	88.8
rps4-trnL-UAA_p3	CTCAGCGCATTACAGGAAGT	ATCTCGTTGGGGATAGAGGG	50.0	55.0	58.0	58.0	1760-1775 bp	0.75	78.4
rps4-trnL-UAA_p4	CTCAGCGCATTACAGGAAGT	GTCCGAGGTTTCCCTGAATC	50.0	55.0	58.0	58.0	1204-1374 bp	0.75	78.4
rps4-trnL-UAA_p5	CTCAGCGCATTACAGGAAGT	GCTGCATTACCAGGTACGAA	50.0	50.0	58.0	58.0	1507 bp	0.25	49.7