Hotspot

psbD-trnT-GGU

Genome · 33984-35401 · 1418 bp

Back to Diplazium

Pi 0.0962

MarkerSeek score 84.20

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1418
variable_sites: 233
pi: 0.0962
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TAATAGGCCAGACCAACCCA	CAGTAGTACGGTGCTTGGTC	50.0	55.0	58.0	58.0	2039-2058 bp	0.75	79.8
psbD-trnT-GGU_p2	TAATAGGCCAGACCAACCCA	GTCAGTAGTACGGTGCTTGG	50.0	55.0	58.0	58.0	2041-2060 bp	0.75	79.8
psbD-trnT-GGU_p3	TTGTACCTGTGAACCAACCG	CAGTAGTACGGTGCTTGGTC	50.0	55.0	58.0	58.0	2088-2107 bp	0.75	79.6
psbD-trnT-GGU_p4	AAGCTATTGCGTTGTAGGGG	CAGTAGTACGGTGCTTGGTC	50.0	55.0	58.0	58.0	2386 bp	0.25	49.6
psbD-trnT-GGU_p5	AAGCTATTGCGTTGTAGGGG	GTCAGTAGTACGGTGCTTGG	50.0	55.0	58.0	58.0	2388 bp	0.25	49.6