Hotspot

trnC-GCA-trnG-GCC

Genome · 29819-30253 · 435 bp

Back to Diplazium

Pi 0.138

MarkerSeek score 84.40

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 435
variable_sites: 102
pi: 0.138
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	TCTTTGCCAAGGAGATGACG	50.0	50.0	57.9	57.9	500-518 bp	1.0	90.4
trnC-GCA-trnG-GCC_p2	TCCGTCTTACCGCTTGACTA	TCTTTGCCAAGGAGATGACG	50.0	50.0	58.2	57.9	488-506 bp	1.0	89.8
trnC-GCA-trnG-GCC_p3	TTTGCAGTCCTCCGTCTTAC	TCTTTGCCAAGGAGATGACG	50.0	50.0	57.5	57.9	498-516 bp	1.0	87.6
trnC-GCA-trnG-GCC_p4	TAAGGATTTGCAGTCCTCCG	TCTTTGCCAAGGAGATGACG	50.0	50.0	57.3	57.9	504-522 bp	1.0	85.7
trnC-GCA-trnG-GCC_p5	CCGTCTTACCGCTTGACTAT	TCTTTGCCAAGGAGATGACG	50.0	50.0	57.1	57.9	487-505 bp	1.0	84.3