Hotspot

psbM-petN

Genome · 28248-29112 · 865 bp

Back to Diplazium

Pi 0.0948

MarkerSeek score 83.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 865
variable_sites: 137
pi: 0.0948
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TTTGCCTCATCGAGTTACCG	ACTTGTAGTTCGGGGAAGGA	50.0	50.0	58.0	58.0	1633 bp	0.25	49.8
psbM-petN_p2	GCCACCGCACTTTTTATTCC	ACTTGTAGTTCGGGGAAGGA	50.0	50.0	58.0	58.0	974 bp	0.25	49.8
psbM-petN_p3	ACACTTCGGTTTTGCCTCAT	ACTTGTAGTTCGGGGAAGGA	45.0	50.0	58.0	58.0	1643 bp	0.25	49.7
psbM-petN_p4	GCCACTCGAGCTATTTTCCA	ACTTGTAGTTCGGGGAAGGA	50.0	50.0	58.0	58.0	1043 bp	0.25	49.6
psbM-petN_p5	TCTTAGCCACCGCACTTTTT	ACTTGTAGTTCGGGGAAGGA	45.0	50.0	58.0	58.0	979 bp	0.25	49.6