Hotspot

trnS-GCU-ycf12

Genome · 7940-8499 · 560 bp

Back to Diplazium

Pi 0.0923

MarkerSeek score 81.70

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 560
variable_sites: 90
pi: 0.0923
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	GTCAAATGATCCCGGACGAA	CACAGATTACCCTTCCGAGC	50.0	55.0	58.0	58.1	855-859 bp	0.5	66.7
trnS-GCU-ycf12_p2	GTCAAATGATCCCGGACGAA	TAGGTATTCGGTTGTGCTGC	50.0	50.0	58.0	58.0	885 bp	0.25	49.7
trnS-GCU-ycf12_p3	GTCAAATGATCCCGGACGAA	TACGACTTCCGAAGCACAAG	50.0	50.0	58.0	57.9	1218 bp	0.25	49.4
trnS-GCU-ycf12_p4	GGGAGAGAGAGGGATTCGAA	TAGGTATTCGGTTGTGCTGC	55.0	50.0	57.9	58.0	775 bp	0.25	49.3
trnS-GCU-ycf12_p5	GTCAAATGATCCCGGACGAA	CGTCACAGATTACCCTTCCG	50.0	55.0	58.0	58.1	862 bp	0.25	49.2