Hotspot

psbK-psbI

Genome · 7131-7670 · 540 bp

Back to Diplazium

Pi 0.112

MarkerSeek score 82.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 540
variable_sites: 105
pi: 0.112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	ACTTCGGGTTTCCTGTCAAC	TTCGTCCGGGATCATTTGAC	50.0	50.0	58.0	58.0	1105-1142 bp	0.5	67.2
psbK-psbI_p2	CACTTCGGGTTTCCTGTCAA	TTCGTCCGGGATCATTTGAC	50.0	50.0	58.0	58.0	1106-1143 bp	0.5	67.2
psbK-psbI_p3	GCGAGAAGAGTTTGGGGTAG	TTCGTCCGGGATCATTTGAC	55.0	50.0	58.0	58.0	1045 bp	0.25	49.8
psbK-psbI_p4	GTAGCGAGAAGAGTTTGGGG	TTCGTCCGGGATCATTTGAC	55.0	50.0	58.0	58.0	1048 bp	0.25	49.8
psbK-psbI_p5	AATCTTCGATCCCGTTGTGG	TTCGTCCGGGATCATTTGAC	50.0	50.0	58.0	58.0	725 bp	0.25	49.7