Hotspot

matK-rps16

Genome · 2965-3584 · 620 bp

Back to Diplazium

Pi 0.0874

MarkerSeek score 79.10

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 620
variable_sites: 100
pi: 0.0874
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CGAGACTGAGGCTGAGTAGA	CTGCGAACGAGGGGATTTTA	55.0	50.0	58.0	58.0	1772 bp	0.25	49.6
matK-rps16_p2	CGAGACTGAGGCTGAGTAGA	TGCGAACGAGGGGATTTTAG	55.0	50.0	58.0	58.0	1771 bp	0.25	49.6
matK-rps16_p3	CGAGACTGAGGCTGAGTAGA	GGAGCGCAATCAACAGAAAC	55.0	50.0	58.0	58.0	1315 bp	0.25	49.5
matK-rps16_p4	CGAGACTGAGGCTGAGTAGA	CTTCGGGGTTAGGCCTAAAC	55.0	55.0	58.0	58.0	1813 bp	0.25	49.5
matK-rps16_p5	CGAGACTGAGGCTGAGTAGA	TTTTAGGGGTCGGTGAGGAT	55.0	50.0	58.0	58.0	1868 bp	0.25	49.4