Hotspot

rpl23

Genome · 82375-82644 · 270 bp

Back to Diplaziopsis

Pi 0.0333

MarkerSeek score 56.70

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 270
variable_sites: 9
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	CCCCGTGGGTGCAAAATATA	CGTGTTACAATACCGTCGGT	50.0	50.0	57.9	58.0	946 bp	0.5	59.4
rpl23_p2	CCCCGTGGGTGCAAAATATA	CTGGAGCTGCTACTTTGGAG	50.0	55.0	57.9	58.0	1391 bp	0.5	59.3
rpl23_p3	ACGTTGCGATATCTCCAACC	CGTGTTACAATACCGTCGGT	50.0	50.0	58.1	58.0	972 bp	0.5	59.3
rpl23_p4	CCCCGTGGGTGCAAAATATA	GATTTTGCTGGCCGTGTTAC	50.0	50.0	57.9	58.0	958 bp	0.5	59.3
rpl23_p5	ACGTTGCGATATCTCCAACC	CTGGAGCTGCTACTTTGGAG	50.0	55.0	58.1	58.0	1417 bp	0.5	59.3