Hotspot

rpl32-trnP-GGG

Genome · 109825-110464 · 640 bp

Back to Diplaziopsis

Pi 0.0549

MarkerSeek score 74.60

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 640
variable_sites: 35
pi: 0.0549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	ACGAACTTCTGGATCCAGGA	CACAAACGCGAAGAGGGATA	50.0	50.0	58.1	58.0	1447 bp	0.5	59.5
rpl32-trnP-GGG_p2	ACGAACTTCTGGATCCAGGA	TATGTAGCGGAATATGGCGC	50.0	50.0	58.1	58.0	893 bp	0.5	59.5
rpl32-trnP-GGG_p3	TTTCATTTACCCCTGCCCAC	CACAAACGCGAAGAGGGATA	50.0	50.0	58.1	58.0	1573 bp	0.5	59.4
rpl32-trnP-GGG_p4	TTTCATTTACCCCTGCCCAC	TATGTAGCGGAATATGGCGC	50.0	50.0	58.1	58.0	1019 bp	0.5	59.4
rpl32-trnP-GGG_p5	ACGAACTTCTGGATCCAGGA	GAGGTCACAGGTTCGAATCC	50.0	55.0	58.1	58.0	843 bp	0.5	59.4