Hotspot

trnR-ACG-rrn5

Genome · 85739-86011 · 273 bp

Back to Diplaziopsis

Pi 0.0476

MarkerSeek score 73.90

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: Genome
length_bp: 273
variable_sites: 13
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	TAGGGATGATTGATTGCGGG	GGTAACAATACTGCAGGGGG	50.0	55.0	57.4	57.9	415-420 bp	1.0	77.5
trnR-ACG-rrn5_p2	TAGGGATGATTGATTGCGGG	GAACCACACCGATCCATCTC	50.0	55.0	57.4	58.1	460-465 bp	1.0	77.2
trnR-ACG-rrn5_p3	TAGGGATGATTGATTGCGGG	CCGATCCATCTCGAACTTGG	50.0	55.0	57.4	58.1	452-457 bp	1.0	76.6
trnR-ACG-rrn5_p4	TAGGGATGATTGATTGCGGG	CGGTAACAATACTGCAGGGG	50.0	55.0	57.4	58.3	416-421 bp	1.0	75.3
trnR-ACG-rrn5_p5	TAGGGATGATTGATTGCGGG	CCTGCGGAAAAATAGCTCGA	50.0	50.0	57.4	58.3	392-397 bp	1.0	75.3