Hotspot

ndhC-trnM-CAU

Genome · 48922-50176 · 1255 bp

Back to Diplaziopsis

Pi 0.0606

MarkerSeek score 78.30

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: Genome
length_bp: 1255
variable_sites: 75
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	GGAGTCATATTGGTGGAGCA	GCGATACTCTAACCGCTGAG	50.0	55.0	57.0	58.0	1312-1423 bp	1.0	76.8
ndhC-trnM-CAU_p2	GGAGTCATATTGGTGGAGCA	TTCGAACCAATGACTCTCGC	50.0	50.0	57.0	58.3	1341-1452 bp	1.0	74.7
ndhC-trnM-CAU_p3	GGAGTCATATTGGTGGAGCAA	GCGATACTCTAACCGCTGAG	47.6	55.0	57.7	58.0	1312-1423 bp	1.0	74.6
ndhC-trnM-CAU_p4	AGGAGTCATATTGGTGGAGC	GCGATACTCTAACCGCTGAG	50.0	55.0	56.7	58.0	1313-1424 bp	1.0	74.4
ndhC-trnM-CAU_p5	GGAGTCATATTGGTGGAGCA	CAATGACTCTCGCCGTATGA	50.0	50.0	57.0	57.5	1334-1445 bp	1.0	73.1