Hotspot

trnL-UAA-trnF-GAA

Genome · 46602-46920 · 319 bp

Back to Diplaziopsis

Pi 0.0579

MarkerSeek score 76.30

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 319
variable_sites: 18
pi: 0.0579
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TGCAGTAGGACGAAAATCCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.0	58.1	433-441 bp	1.0	84.3
trnL-UAA-trnF-GAA_p2	GCAGTAGGACGAAAATCCGT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.0	58.1	432-440 bp	1.0	84.3
trnL-UAA-trnF-GAA_p3	ATCCGTTGGTTTCACAGGAC	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	418-426 bp	1.0	83.7
trnL-UAA-trnF-GAA_p4	AGGACGAAAATCCGTTGGTT	TTTGAACTGGTGACACGAGG	45.0	50.0	57.7	58.1	427-435 bp	1.0	82.3
trnL-UAA-trnF-GAA_p5	TGCAGTAGGACGAAAATCCG	TGGCCTATCTCCCAAACCAT	50.0	50.0	58.0	58.4	472-480 bp	1.0	81.2