Hotspot

rps4-trnL-UAA

Genome · 45217-45920 · 704 bp

Back to Diplaziopsis

Pi 0.0512

MarkerSeek score 78.10

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 704
variable_sites: 35
pi: 0.0512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GAGGTCCTCGGTAGCGAG	ATTGCCCGATAAATGGTCCC	66.7	50.0	58.6	58.0	838-842 bp	1.0	64.6
rps4-trnL-UAA_p2	GAGGTCCTCGGTAGCGAG	GTCTCCGTACCTAGGCTCAA	66.7	55.0	58.6	58.2	783-787 bp	1.0	63.1
rps4-trnL-UAA_p3	TCAATTTCAAACGAGGTCCTC	ATTGCCCGATAAATGGTCCC	42.9	50.0	56.2	58.0	850-854 bp	1.0	62.6
rps4-trnL-UAA_p4	GAGGTCCTCGGTAGCGAG	GGCTGCCACTTTATACGTCT	66.7	50.0	58.6	57.7	799-803 bp	1.0	62.4
rps4-trnL-UAA_p5	GGTCCTCGGTAGCGAGAC	ATTGCCCGATAAATGGTCCC	66.7	50.0	58.9	58.0	836-840 bp	1.0	62.1