Hotspot

psbM-petN

Genome · 28410-29688 · 1279 bp

Back to Diplaziopsis

Pi 0.0454

MarkerSeek score 71.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1279
variable_sites: 57
pi: 0.0454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCCACCGCACTTTTTATTCC	TCAAGCTTTGTCTTGTGCCT	50.0	45.0	58.0	57.9	1519-1529 bp	1.0	83.9
psbM-petN_p2	GCCACCGCACTTTTTATTCC	AGGTTGAGGTGCTCCATAGT	50.0	50.0	58.0	58.0	1776-1786 bp	1.0	83.8
psbM-petN_p3	GCCACCGCACTTTTTATTCC	TTTCATGTGTCTCGATGCGT	50.0	45.0	58.0	57.9	1738-1748 bp	1.0	83.6
psbM-petN_p4	GCCACCGCACTTTTTATTCC	CTACACGTCGACGACATACC	50.0	55.0	58.0	57.9	2071-2079 bp	1.0	83.4
psbM-petN_p5	ATTGGGTAGGAATCGCCAAC	AGGTTGAGGTGCTCCATAGT	50.0	50.0	57.9	58.0	2413-2416 bp	1.0	83.2