Hotspot

rpoB-trnD-GUC

Genome · 26644-27428 · 785 bp

Back to Diplaziopsis

Pi 0.0744
MarkerSeek score 78.50
Primer available yes
Label rpoB-trnD-GUC

Feature Metadata

label_name
rpoB-trnD-GUC
feature_type
igs
region
Genome
length_bp
785
variable_sites
58
pi
0.0744
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnD-GUC_p1 CAAGTTCTGGTAGTACGGGC TTATCGGGATTGACGGGTCT 55.0 50.0 58.0 58.2 885-903 bp 1.0 83.9
rpoB-trnD-GUC_p2 GCAAGTTCTGGTAGTACGGG TTATCGGGATTGACGGGTCT 55.0 50.0 58.0 58.2 886-904 bp 1.0 83.9
rpoB-trnD-GUC_p3 AGTTCTGGTAGTACGGGCAT TTATCGGGATTGACGGGTCT 50.0 50.0 58.1 58.2 883-901 bp 1.0 82.8
rpoB-trnD-GUC_p4 CAAGTTCTGGTAGTACGGGC GCGGTGCTCTAACCAATTGA 55.0 50.0 58.0 58.6 838-856 bp 1.0 81.5
rpoB-trnD-GUC_p5 GCAAGTTCTGGTAGTACGGG GCGGTGCTCTAACCAATTGA 55.0 50.0 58.0 58.6 839-857 bp 1.0 81.5