Hotspot

petA-psbJ

LSC · 63342-64329 · 988 bp

Back to Diplacus

Pi 0.0038

MarkerSeek score 52.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 11
pi: 0.0038
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1124-1127 bp	1.0	73.8
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.6	57.4	1123-1126 bp	1.0	73.3
petA-psbJ_p3	TCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1130-1133 bp	1.0	71.0
petA-psbJ_p4	CCATTACGTGTCCAAGGTCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1129-1132 bp	1.0	71.0
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	CCGGATTAGGTTCATCCCTG	50.0	55.0	57.2	57.4	1129-1132 bp	1.0	70.5