Hotspot

psbZ-trnG-GCC

LSC · 36689-36963 · 275 bp

Back to Diplacus

Pi 0.00714

MarkerSeek score 49.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 5
pi: 0.00714
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	443-464 bp	1.0	79.0
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	444-465 bp	1.0	79.0
psbZ-trnG-GCC_p3	GCTTCTCCTGATGGTTGGTC	AATCGAACCCGCATCTTCTC	55.0	50.0	58.3	58.1	441-462 bp	1.0	78.5
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCATCTTCT	55.0	50.0	58.3	58.1	442-463 bp	1.0	78.5
psbZ-trnG-GCC_p5	CCGTCGTATTTGCTTCTCCT	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	452-473 bp	1.0	78.2