Hotspot

psbM-trnD-GUC

LSC · 30469-30993 · 525 bp

Back to Diplacus

Pi 0.00129

MarkerSeek score 50.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 525
variable_sites: 2
pi: 0.00129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	641-656 bp	1.0	70.2
psbM-trnD-GUC_p2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.9	58.5	645-660 bp	1.0	69.6
psbM-trnD-GUC_p3	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.2	58.5	648-663 bp	1.0	68.9
psbM-trnD-GUC_p4	AGTAGAAAGGCGGTAGGAAC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	648-663 bp	1.0	67.6
psbM-trnD-GUC_p5	GTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.7	58.5	647-662 bp	1.0	67.6