Hotspot

trnK-UUU-rps16

LSC · 4503-5410 · 908 bp

Back to Diplacus

Pi 0.00586
MarkerSeek score 59.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
908
variable_sites
15
pi
0.00586
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1024-1070 bp 1.0 68.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1023-1069 bp 1.0 68.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 1038-1084 bp 1.0 67.7
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1023-1069 bp 1.0 67.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1022-1068 bp 1.0 67.7