Hotspot

trnH-GUG-psbA

LSC · 93-623 · 531 bp

Back to Diplacus

Pi 0.00983

MarkerSeek score 58.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 12
pi: 0.00983
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	597-656 bp	1.0	82.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	611-670 bp	1.0	82.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	610-669 bp	1.0	82.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	612-671 bp	1.0	82.1
trnH-GUG-psbA_p5	TTTTGTTTTCTGCATGGGCG	TTCCCTCTAGACCTAGCTGC	45.0	55.0	58.1	57.9	666-725 bp	1.0	81.8