Hotspot

ndhH

SSC · 124783-125964 · 1182 bp

Back to Diphylleia

Pi 0.00451

MarkerSeek score 61.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 8
pi: 0.00451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ACAAGCAACCATATGAGTCCA	CGAGAGCCAAAAACTCGTCT	42.9	50.0	57.3	58.2	1357 bp	1.0	65.0
ndhH_p2	AACAAGCAACCATATGAGTCCA	CGAGAGCCAAAAACTCGTCT	40.9	50.0	58.0	58.2	1358 bp	1.0	62.5
ndhH_p3	ACAAGCAACCATATGAGTCCAT	CGAGAGCCAAAAACTCGTCT	40.9	50.0	57.8	58.2	1357 bp	1.0	60.8
ndhH_p4	AGGAACAAGCAACCATATGAGT	CGAGAGCCAAAAACTCGTCT	40.9	50.0	57.7	58.2	1361 bp	1.0	60.3
ndhH_p5	ACAAGCAACCATATGAGTCCA	CGAGAGCCAAAAACTCGTCTA	42.9	47.6	57.3	58.1	1357 bp	1.0	58.3