Hotspot

trnV-GAC-rps12

IRa · 141272-142791 · 1520 bp

Back to Diphylleia

Pi 0.00154

MarkerSeek score 61.60

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1520
variable_sites: 3
pi: 0.00154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.9	57.7	1652-1677 bp	1.0	78.3
trnV-GAC-rps12_p2	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1576-1601 bp	1.0	76.6
trnV-GAC-rps12_p3	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.4	57.7	1601-1626 bp	1.0	76.5
trnV-GAC-rps12_p4	GCTCGAACTGATGACTTCCA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	1600-1625 bp	1.0	76.4
trnV-GAC-rps12_p5	CAGGCTCGAACTGATGACTT	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	1603-1628 bp	1.0	76.4