Hotspot

ndhF-rpl32

SSC · 115362-116234 · 873 bp

Back to Diphylleia

Pi 0.0179

MarkerSeek score 65.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 873
variable_sites: 23
pi: 0.0179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGGAAGGAAGGGTATGATC	GTACGTTTCTTTGGAACCGC	55.0	50.0	57.8	57.7	921-942 bp	1.0	81.6
ndhF-rpl32_p2	AGCGGAAGGAAGGGTATGAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.2	57.7	922-943 bp	1.0	81.4
ndhF-rpl32_p3	AGTCCCACTCCGATTGCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.2	57.7	952-973 bp	1.0	81.1
ndhF-rpl32_p4	TTGCTATAGGAACTGGCAGC	GTACGTTTCTTTGGAACCGC	50.0	50.0	57.7	57.7	939-960 bp	1.0	80.2
ndhF-rpl32_p5	AAGTCCCACTCCGATTGCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.4	57.7	953-974 bp	1.0	79.3