Hotspot

rps12-trnV-GAC

IRb · 101294-102813 · 1520 bp

Back to Diphylleia

Pi 0.00154

MarkerSeek score 61.60

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1520
variable_sites: 3
pi: 0.00154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	45.0	50.0	57.7	57.9	1652-1677 bp	1.0	78.3
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	45.0	55.0	57.7	57.7	1576-1601 bp	1.0	76.6
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	GGCTCGAACTGATGACTTCC	45.0	55.0	57.7	58.4	1601-1626 bp	1.0	76.5
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	GCTCGAACTGATGACTTCCA	45.0	50.0	57.7	57.6	1600-1625 bp	1.0	76.4
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	CAGGCTCGAACTGATGACTT	45.0	50.0	57.7	57.6	1603-1628 bp	1.0	76.4