Hotspot

petA-psbJ

LSC · 65647-66830 · 1184 bp

Back to Diphylleia

Pi 0.0107

MarkerSeek score 56.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 19
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	36.4	50.0	55.8	57.6	1254-1264 bp	1.0	44.8
petA-psbJ_p2	AAGAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	34.8	50.0	56.5	57.6	1255-1265 bp	1.0	42.5
petA-psbJ_p3	AGAAACAGTTTGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	34.8	50.0	56.5	57.6	1254-1264 bp	1.0	42.5
petA-psbJ_p4	GAAACAGTTTGAGAAGGTTCA	ATTCCGGATTGGGTTCATCC	38.1	50.0	54.3	57.6	1253-1263 bp	1.0	41.4
petA-psbJ_p5	AAGAAACAGTTTGAGAAGGTTCAA	ATTCCGGATTGGGTTCATCC	33.3	50.0	57.2	57.6	1255-1265 bp	1.0	41.4