Hotspot

accD-psaI

LSC · 61828-62497 · 670 bp

Back to Diphylleia

Pi 0.00707

MarkerSeek score 61.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 7
pi: 0.00707
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGTGTTCTGAGCGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	785-801 bp	1.0	77.1
accD-psaI_p2	AAAGGTGTTCTGAGCGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	784-800 bp	1.0	77.1
accD-psaI_p3	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	811-827 bp	1.0	76.8
accD-psaI_p4	AAAAGGTGTTCTGAGCGAGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.4	57.9	791-807 bp	1.0	76.5
accD-psaI_p5	AAAGGTGTTCTGAGCGAGTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.4	57.9	790-806 bp	1.0	76.5