Hotspot

ndhC-trnV-UAC

LSC · 52830-54453 · 1624 bp

Back to Diphylleia

Pi 0.0199

MarkerSeek score 62.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1624
variable_sites: 46
pi: 0.0199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	1609-1680 bp	1.0	53.6
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	1608-1679 bp	1.0	50.8
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1597-1668 bp	1.0	49.7
ndhC-trnV-UAC_p4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	1608-1679 bp	1.0	49.6
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.6	57.8	1609-1680 bp	1.0	49.5