Hotspot

trnS-UGA-psbZ

LSC · 37528-37877 · 350 bp

Back to Diphylleia

Pi 0.0086

MarkerSeek score 52.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 350
variable_sites: 4
pi: 0.0086
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	ACTAGACCAACCCTCAGGAG	55.0	55.0	58.1	57.8	489-491 bp	1.0	78.6
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	482-484 bp	1.0	78.3
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	ACTAGACCAACCCTCAGGAG	55.0	55.0	58.1	57.8	478-480 bp	1.0	78.1
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	471-473 bp	1.0	77.8
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	AGACCAACCCTCAGGAGAAG	55.0	55.0	58.1	58.3	486-488 bp	1.0	77.7