Hotspot

trnH-GUG-psbA

LSC · 76-654 · 579 bp

Back to Diphylleia

Pi 0.0364

MarkerSeek score 75.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 29
pi: 0.0364
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	692-703 bp	1.0	86.0
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	693-704 bp	1.0	85.8
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	TATCATCAACCGCGCTAACC	50.0	50.0	57.9	58.1	703-714 bp	1.0	85.1
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TATCATCAACCGCGCTAACC	50.0	50.0	58.2	58.1	704-715 bp	1.0	84.9
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	CTGATATCATCAACCGCGCT	50.0	50.0	57.9	58.2	707-718 bp	1.0	84.5