Hotspot

ycf2

IRa · 148023-154796 · 6774 bp

Back to Dipelta

Pi 0.0012

MarkerSeek score 39.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6774
variable_sites: 8
pi: 0.0012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGGAAGTATCATGATCGTCCA	TCCGATTGATTGGTCTGAAGT	47.6	42.9	57.0	57.0	314-317 bp	1.0	49.9
ycf2_p2	TGGGAAGTATCATGATCGTCC	TCCGATTGATTGGTCTGAAGT	47.6	42.9	57.0	57.0	315-318 bp	1.0	49.9
ycf2_p3	GGGAAGTATCATGATCGTCCA	TCCGATTGATTGGTCTGAAGTT	47.6	40.9	57.0	57.7	314-317 bp	1.0	47.5
ycf2_p4	TGGGAAGTATCATGATCGTCC	TCCGATTGATTGGTCTGAAGTT	47.6	40.9	57.0	57.7	315-318 bp	1.0	47.5
ycf2_p5	GGGAAGTATCATGATCGTCCAA	TCCGATTGATTGGTCTGAAGT	45.5	42.9	57.7	57.0	314-317 bp	1.0	47.5