Hotspot

rpl16

LSC · 84695-85105 · 411 bp

Back to Dipelta

Pi 0

MarkerSeek score 22.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGCATATCGGCGATTGCTAG	GACGGAACCTGTGAATCCAA	50.0	50.0	58.0	57.8	1245-1258 bp	1.0	79.6
rpl16_p2	CATATCGGCGATTGCTAGCT	GACGGAACCTGTGAATCCAA	50.0	50.0	58.0	57.8	1243-1256 bp	1.0	79.6
rpl16_p3	GAGCATATCGGCGATTGCTA	GACGGAACCTGTGAATCCAA	50.0	50.0	58.0	57.8	1246-1259 bp	1.0	79.6
rpl16_p4	AGCATATCGGCGATTGCTAG	TCACGTCCGGTTCTGTAGTA	50.0	50.0	58.0	57.8	733 bp	1.0	78.7
rpl16_p5	CATATCGGCGATTGCTAGCT	TCACGTCCGGTTCTGTAGTA	50.0	50.0	58.0	57.8	731 bp	1.0	78.7