Hotspot

ycf2-trnI-CAU

IRa · 154797-154980 · 184 bp

Back to Dipelta

Pi 0.105

MarkerSeek score 74.10

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 184
variable_sites: 19
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.9	58.0	422-447 bp	1.0	85.2
ycf2-trnI-CAU_p2	GTTGAACCCTGTGAATTGCG	ATTGGCATGCATCCAGTAGG	50.0	50.0	57.9	58.0	471-496 bp	1.0	84.7
ycf2-trnI-CAU_p3	TATCGTTTCTGGGCCCAATG	ATTGGCATGCATCCAGTAGG	50.0	50.0	57.9	58.0	626-651 bp	1.0	83.4
ycf2-trnI-CAU_p4	CCCTCTTTGTTCCGATCCAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.0	58.0	781-806 bp	1.0	82.8
ycf2-trnI-CAU_p5	CCCTCTTTGTTCCGATCCAG	ATTGGCATGCATCCAGTAGG	55.0	50.0	58.0	58.0	830-855 bp	1.0	82.6