Hotspot

rpl32-trnL-UAG

SSC · 116300-117093 · 794 bp

Back to Dipelta

Pi 0.00517
MarkerSeek score 64.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
794
variable_sites
4
pi
0.00517
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGTCGGCGTTAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 899-920 bp 1.0 80.2
rpl32-trnL-UAG_p2 GGGATATTGGTCGGCGTTAA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 900-921 bp 1.0 80.2
rpl32-trnL-UAG_p3 GGTCGGCGTTAAAAGCTTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 891-912 bp 1.0 79.6
rpl32-trnL-UAG_p4 GGTCGGCGTTAAAAGCTTTG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 892-913 bp 1.0 79.6
rpl32-trnL-UAG_p5 GGGATATTGGTCGGCGTTAA TCTTTTAGAAGACGGCCTGC 50.0 50.0 57.8 57.9 967-988 bp 1.0 79.3