Hotspot

rps7-rps12

IRb · 101392-101444 · 53 bp

Back to Dipelta

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TGCAGTACCTCTACGTGACA	GGCTTGGAGGGAGATCTTTC	50.0	55.0	58.1	57.7	143 bp	1.0	77.0
rps7-rps12_p2	TGCAGTACCTCTACGTGACA	ATCGTTCGAGATCCACCCTA	50.0	50.0	58.1	57.6	121 bp	1.0	77.0
rps7-rps12_p3	TGCAGTACCTCTACGTGACA	TATCGTTCGAGATCCACCCT	50.0	50.0	58.1	57.6	122 bp	1.0	77.0
rps7-rps12_p4	TGCAGTACCTCTACGTGACA	TATGTACGGCTTGGAGGGAG	50.0	55.0	58.1	58.6	150 bp	1.0	75.0
rps7-rps12_p5	TGCAGTACCTCTACGTGACA	TCGTTCGAGATCCACCCTAC	50.0	55.0	58.1	58.6	120 bp	1.0	75.0