Hotspot

trnI-CAU-ycf2

IRb · 89953-90136 · 184 bp

Back to Dipelta

Pi 0.105

MarkerSeek score 74.10

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 184
variable_sites: 19
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	422-447 bp	1.0	85.2
trnI-CAU-ycf2_p2	ATTGGCATGCATCCAGTAGG	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	471-496 bp	1.0	84.7
trnI-CAU-ycf2_p3	ATTGGCATGCATCCAGTAGG	TATCGTTTCTGGGCCCAATG	50.0	50.0	58.0	57.9	626-651 bp	1.0	83.4
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	CCCTCTTTGTTCCGATCCAG	50.0	55.0	58.0	58.0	781-806 bp	1.0	82.8
trnI-CAU-ycf2_p5	ATTGGCATGCATCCAGTAGG	CCCTCTTTGTTCCGATCCAG	50.0	55.0	58.0	58.0	830-855 bp	1.0	82.6