Hotspot

rpl20-rps12

LSC · 73462-74261 · 800 bp

Back to Dipelta

Pi 0.00129

MarkerSeek score 62.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	894-919 bp	1.0	75.5
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	893-918 bp	1.0	75.5
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	893-918 bp	1.0	74.8
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	892-917 bp	1.0	74.8
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	TCAGCGTCGAGGAACATGTA	50.0	50.0	57.9	58.8	894-919 bp	1.0	74.7