Hotspot

petA-psbJ

LSC · 66860-67866 · 1007 bp

Back to Dipelta

Pi 0.0321

MarkerSeek score 78.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 32
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GGGTCATATTCCGGATTGGG	50.0	55.0	57.8	57.8	1133-1142 bp	1.0	80.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1126-1135 bp	1.0	78.7
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGGTCATATTCCGGATTGGG	50.0	55.0	58.7	57.8	1140-1149 bp	1.0	75.8
petA-psbJ_p4	GATCCATTACGCGTCCAAGG	GGGTCATATTCCGGATTGGG	55.0	55.0	58.8	57.8	1151-1160 bp	1.0	75.0
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1133-1142 bp	1.0	74.5