Hotspot

rbcL-accD

LSC · 58410-60139 · 1730 bp

Back to Dipelta

Pi 0.0568

MarkerSeek score 90.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1730
variable_sites: 96
pi: 0.0568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 90.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TAAACAGCTGTCTGGGGGTA	TCAAGGATGGATCTAGGGGG	50.0	55.0	58.0	57.6	615-633 bp	1.0	84.5
rbcL-accD_p2	CATTCGGAGTTGCGATGAGA	TCAAGGATGGATCTAGGGGG	50.0	55.0	58.1	57.6	243-261 bp	1.0	84.3
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	AAAGAGGACTCCACTTCGGA	50.0	50.0	57.9	58.0	2960-2969 bp	1.0	83.5
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	GGACTCCACTTCGAAATCCC	50.0	55.0	57.9	58.0	2919-2928 bp	1.0	83.5
rbcL-accD_p5	AATGAGGGACGTGATCTTGC	GGACTCCACTTCGAAATCCC	50.0	55.0	58.0	58.0	2994 bp	0.5	59.6