Hotspot

trnS-GCU-trnG-GCC

LSC · 8904-9685 · 782 bp

Back to Dipelta

Pi 0.00264

MarkerSeek score 63.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 2
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	45.0	40.9	58.0	57.7	886-901 bp	1.0	63.6
trnS-GCU-trnG-GCC_p2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.1	57.7	899-914 bp	1.0	63.2
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.2	57.7	935-950 bp	1.0	62.6
trnS-GCU-trnG-GCC_p4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.8	57.7	900-915 bp	1.0	62.2
trnS-GCU-trnG-GCC_p5	TAGTCCACTCAGCCATCTCC	TGAATCAAACCGAAAGATCCCT	55.0	40.9	58.2	57.7	868-883 bp	1.0	62.2